《植物生理学报》 2015, 51(4): 559-565

水杨酸诱导膨胀浮萍(Lemna gibba SH0204)开花

黄猛^1,2, 许亚良², Kanjana Khaeso², 孙雪飘², 张家明^2,*

¹海南大学农学院, 海口570228; ²中国热带农业科学院热带生物技术研究所, 农业部热带作物生物学与遗传资源利用重点实验室, 海南省热带生物能源工程技术研究中心, 海口571101

通信作者：张家明；E-mail: jmzhang@vip.163.com；Tel: 0898-66984866

摘　要：

用水杨酸(SA)诱导膨胀浮萍开花, 结果显示, 在液体1/2Hoagland培养基中加入适当浓度的SA对开花有显著促进作用, SA浓度为20 μmol•L^-1时开花率最高, 达到39.37%。SA在诱导开花的同时, 明显抑制叶状体的生长, 在浓度为80 μmol•L^-1时, 叶状体死亡率达到94.38%。此外, 本研究尝试在固体1/2Hoagland培养基上诱导膨胀浮萍开花, 结果表明, 固体培养基比液体培养基更有利于诱导开花, SA浓度为20 μmol•L^-1时, 开花率超过45%, 死亡率显著降低。在固体培养基上, 方便对开花植株进行跟踪和操作, 因此, 用固体培养基诱导开花比液体培养基更有应用价值。光学和电子显微镜的观察结果显示, 花粉粒呈近圆球形, 表面有网状带刺纹饰。

关键词：水杨酸; 膨胀浮萍; 开花诱导; 花粉粒

收稿：2015-01-04   修定：2015-03-25

资助：科技部国际合作专项(2014DFA30680)、2014年海南省重大科技专项(ZDZX2013023)和海南省自然科学基金项目(310068)。

Flower-Induction of Lemna gibba SH0204 by Salicylic Acid

HUANG Meng^1,2, XU Ya-Liang², Kanjana Khaeso², SUN Xue-Piao², ZHANG Jia-Ming^2,*

¹College of Agronomy, Hainan University, Haikou 570228, China; ²Key Laboratory for Tropical Crops Biology and Genetic Resources Utilization, Hainan Bioenergy Engineering Center, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China

Corresponding author: ZHANG Jia-Ming; E-mail: jmzhang@vip.163.com; Tel: 0898-66984866

Abstract:

Salicylic acid (SA) was used in the flower induction of the duckweed Lemna gibba SH0204. Flowers were induced efficiently by suitable concentrations of SA in the liquid 1/2Hoagland medium, and the highest induction rate reached 39.37% at 20 μmol•L^-1 SA. In the meantime, SA inhibited the growth of L. gibba, and the frond mortality reached 94.38% at 80 μmol•L^-1 SA. Solid medium was used in the flower induction of duckweeds, and was found to be more optimal compared to liquid medium. The highest flower induction rate reached 45% on the solid medium, and the frond mortality decreased significantly. Moreover, the fronds were kept in the original locations on the solid medium, avoiding the damage of the flowers on the liquid medium by moving around, and providing convenience of tracing or operation with the flowers. Therefore, solid medium is a practically more useful medium in flower induction of duckweeds. Electron and light microscopic observation revealed that the pollen grains were spheroid and small, with echinate-reticulate exine.

Key words: salicylic acid (SA); Lemna gibba SH0204; flower induction; pollen grains